Gene Targeting by Homology-Directed Repair in Rice Using a Geminivirus-Based CRISPR/Cas9 System.
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منابع مشابه
Gene Targeting by Homology-Directed Repair inRice Using a Geminivirus-Based CRISPR/Cas9 System
Rice (Oryza sativa) is the staple food for more than half of the world’s population. Technologies enabling precise and efficient DNA knock-in or replacement, hereinafter referred to as KI, have the potential to revolutionize the generation of crops by precision molecular breeding. Clustered regularly interspaced short palindromic repeats (CRISPR)-associated Cas9 (CRISPR/Cas9) has recently emerg...
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The CRISPR/Cas9 genome-editing platform is a versatile and powerful technology to efficiently create genetically engineered living cells and organisms. This system requires a complex of Cas9 endonuclease protein with a gene-targeting guide RNA (gRNA) to introduce double-strand DNA breaks (DSBs) at specific locations in the genome. The cell then repairs the resulting DSBs using either homology-d...
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عنوان ژورنال:
- Molecular plant
دوره 10 7 شماره
صفحات -
تاریخ انتشار 2017